Bidirectional Enhancement of Cell Proliferation Between Iron Oxide Nanoparticle-Labeled Mesenchymal Stem Cells and Choroid Plexus in a Cell-Based Therapy Model of Ischemic Stroke

International Journal of Nanomedicine, 2020 · DOI: http://doi.org/10.2147/IJN.S278687 · Published: January 1, 2020

Neurology Biomedical Regenerative Medicine & Stem Cells

Simple Explanation

This study investigates the interaction between mesenchymal stem cells (MSCs) and the choroid plexus (CP) in a rat stroke model. MSCs were labeled with iron oxide nanoparticles (IONs) and injected into the brains of rats with ischemic stroke. The study found that MSCs migrated to the CP and that there was a bidirectional enhancement of proliferation between MSCs and CP.

Study Duration

35 days

Participants

Male rats aged 6–8 weeks

Evidence Level

Not specified

Key Findings

1
ION-labeled MSCs migrate to the CP in an ischemic stroke model, as revealed by MRI and histological analysis.
2
Co-culture of MSCs and CP results in a bidirectional enhancement of proliferation, indicating a crosstalk between the two cell types.
3
Cytokine analysis revealed elevated levels of proliferation- and adhesion-related cytokines and chemokines in the co-culture medium.

Research Summary

The study investigated the interaction between MSCs and CP in a rat stroke model, finding that MSCs migrate to the CP and enhance its proliferation. MRI reveals MSC migration towards the CP in an ischemic stroke model. Bidirectional effects on proliferation were observed in MSC and CP co-cultures involving various cytokines and the IGF-1/Akt, chemokine signaling, and spinal cord injury signaling pathways.

Practical Implications

Cell Therapy Strategies

The findings suggest a shift in cell therapy strategies for stroke from intravenous delivery of MSCs to direct injection into lateral ventricles harboring the CP, which could enhance functional recovery.

Therapeutic Potential

The secreted factors resulting from the interaction between MSCs and CP have therapeutic potential for promoting functional recovery in the brain after ischemic stroke.

Understanding CP Regeneration

This study contributes to the understanding of the mechanisms underlying CP regeneration after stroke.

Bidirectional Enhancement of Cell Proliferation Between Iron Oxide Nanoparticle-Labeled Mesenchymal Stem Cells and Choroid Plexus in a Cell-Based Therapy Model of Ischemic Stroke

Simple Explanation

Key Findings

Research Summary

Practical Implications

Cell Therapy Strategies

Therapeutic Potential

Understanding CP Regeneration

Study Limitations

Your Feedback

Was this summary helpful?

Bidirectional Enhancement of Cell Proliferation Between Iron Oxide Nanoparticle-Labeled Mesenchymal Stem Cells and Choroid Plexus in a Cell-Based Therapy Model of Ischemic Stroke

Simple Explanation

Key Findings

Research Summary

Practical Implications

Cell Therapy Strategies

Therapeutic Potential

Understanding CP Regeneration

Study Limitations

Your Feedback

Was this summary helpful?