Fabrication of chitosan-polyvinyl alcohol and silk electrospun fiber seeded with differentiated keratinocyte for skin tissue regeneration in animal wound model

This study explores creating a skin substitute using a mix of materials (chitosan, polyvinyl alcohol, and silk) formed into tiny fibers via a process called electrospinning. These fibers are then seeded with skin cells (keratinocytes) grown from stem cells to help heal wounds. The goal is to make a material that supports cell growth and helps repair damaged skin.

• 1
  The Ch-PVA + Silk hybrid fiber prominently showed superior mechanical properties and desire swelling as well as hydrophilic microenvironment to those of pure PVA and Ch-PVA fibers.
• 2
  Incorporation of blended Ch and co-electrospun silk in PVA based fibrous mat presented excellent cell adhesion and proliferation in comparison to the neat PVA and Ch-PVA fibers.
• 3
  The in vivo study showed that composite Ch-PVA + Silk fibrous mat in presence of MSC-derived keratinocytes could stimulate wound healing and skin tissue regeneration.