Identification of Prognostic and Metastatic Alternative Splicing Signatures in Kidney Renal Clear Cell Carcinoma

This study explores the role of alternative splicing events (ASEs) in the development and spread (metastasis) of kidney renal clear cell carcinoma (KIRC). The aim of this study is to explore the mechanism of alternative splicing events (ASEs) underlying tumorigenesis and metastasis of KIRC. Researchers analyzed RNA sequencing data from KIRC samples to identify ASEs linked to patient survival and metastasis. Additionally, metastasis-related ASEs along with corresponding SFs and pathways were also identified by Pearson correlation analysis to illuminate the underlying mechanism of metastasis in KIRC. The study identified specific splicing factors and genes (RHOT2, TCIRG1) that may play a role in KIRC metastasis, which could lead to new treatment targets. Aberrant DDX39B regulated RHOT2-32938-RI and TCIRG1-17288-RI might be related to the tumorigenesis, metastasis and poor prognosis of KIRC via sphingolipid metabolism or N-glycan biosynthesis pathway.

• 1
  Identified 6,081 overall survival-related ASEs (OS-SEs) in KIRC. A total of prognostic 6,081 overall survival-related ASEs (OS-SEs) were identified by univariate Cox regression analysis
• 2
  Developed a prediction model based on 5 OS-SEs with an AUC of 0.788, showing good reliability. a prediction model was constructed based on 5 OS-SEs screened by Lasso regression with the Area Under Curve of 0.788.
• 3
  DDX39B was identified as a significant splicing factor correlated with OS and metastasis. Among 390 identified candidate SFs, DExD-Box Helicase 39B (DDX39B) was significantly correlated with OS and metastasis.