Peripheral Nerve Transplantation Combined with Acidic Fibroblast Growth Factor and Chondroitinase Induces Regeneration and Improves Urinary Function in Complete Spinal Cord Transected Adult Mice

PLoS ONE, 2015 · DOI: 10.1371/journal.pone.0139335 · Published: October 1, 2015

Spinal Cord Injury Neurology Regenerative Medicine & Stem Cells

Simple Explanation

This study investigates a treatment for urinary dysfunction following spinal cord injury in mice, using peripheral nerve grafts, acidic fibroblast growth factor, and chondroitinase ABC. The treatment aims to promote nerve regeneration across the injury site to restore bladder control. Results showed improved bladder function, reduced scarring, and nerve regeneration with the combined treatment.

Study Duration

18 weeks post-SCI

Participants

37 adult female C57BL/6 mice

Evidence Level

Not specified

Key Findings

1
The combination of PNG+aFGF+ChABC reduced bladder weight and improved bladder and external urethral sphincter histology.
2
Treatment with PNG+aFGF+ChABC significantly enhanced lower urinary tract function, resulting in more efficient voiding.
3
Injured spinal cords of treated mice showed a reduction in collagen scarring and regeneration of serotonergic and tyrosine hydroxylase-positive axons.

Research Summary

This study demonstrates the effectiveness of a combinatorial therapy (PNG+aFGF+ChABC) in improving lower urinary tract function in mice with complete spinal cord transection. The treatment led to reduced bladder hyperactivity, improved bladder voiding efficiency, and regeneration of key neuronal fibers across the injury site. The findings suggest that this approach can be translated for further investigation into genetic contributions to regeneration failure following spinal cord injury.

Practical Implications

Therapeutic Potential

The combinatorial therapy shows promise for treating urinary dysfunction following spinal cord injury.

Model for Genetic Studies

The mouse model can be used to investigate genetic factors influencing regeneration failure.

Multimodal Approach

The study supports the need to target multiple factors in treating severe spinal cord injuries.

Study Limitations

1
The nerve regeneration effects in mice were not as strong as in previous rat studies.
2
The interface between the PNG and host tissues was not as striking as in rat studies.
3
Technical difficulties in stabilizing the vertebral column in mice may have hindered regeneration.

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