Spinal cord RNA-seq data after a baclofen treatment in mice with a spinal cord injury

Data in Brief, 2023 · DOI: 10.1016/j.dib.2022.108809 · Published: February 1, 2023

Spinal Cord Injury Neurology Bioinformatics

Simple Explanation

This study provides transcriptomic data from adult mouse spinal cords collected after a compression spinal cord injury (SCI) and baclofen administration. The data includes information on gene expression changes and functional annotations. Baclofen is a drug that has shown potential in promoting functional recovery after SCI in mice. This research aims to understand how baclofen affects the spinal cord at a molecular level after injury. The researchers used RNA sequencing to analyze the genes that are turned on or off in the spinal cord after injury and baclofen treatment. This data can help identify pathways involved in regeneration and neuroprotection.

Study Duration

7 days

Participants

12 adult (8 to 12 weeks of age) C57BL/6J female mice

Evidence Level

Not specified

Key Findings

1
The study identified 992 differentially expressed genes in the spinal cord after baclofen treatment compared to a control group. This suggests that baclofen has a significant impact on gene expression following SCI.
2
Of the differentially expressed genes, 347 showed decreased expression, and 645 showed increased expression after baclofen treatment. This indicates that baclofen can both suppress and activate gene expression in the injured spinal cord.
3
Enrichment analyses revealed 95 significantly enriched terms, including GO terms, KEGG pathways and InterPro domains. These enriched terms provide insights into the biological processes and pathways affected by baclofen treatment after SCI.

Research Summary

This data article presents RNA-seq data from a study investigating the effects of baclofen treatment on the spinal cord after a compression injury in mice. The data includes raw sequencing reads and bioinformatic analyses such as differential gene expression and functional enrichment. The study identified a significant number of differentially expressed genes and enriched pathways in the spinal cord of baclofen-treated mice compared to controls. These findings contribute to understanding the molecular mechanisms underlying baclofen's potential to promote functional recovery after SCI. The data is publicly available in the NCBI Sequence Read Archive and Mendeley Data, providing a valuable resource for researchers interested in spinal cord injury, neuroprotection, and regeneration.

Practical Implications

Understanding Mechanisms of Baclofen

The RNA-seq data provides insights into the genetic and cellular effects of baclofen treatment after SCI, helping to elucidate the mechanisms by which baclofen promotes functional recovery.

Target Identification for Therapy

The identified differentially expressed genes and enriched pathways can guide future research and the development of targeted therapies for SCI.

Data Resource for Further Analysis

The publicly available RNA-seq raw data allows researchers to perform their own bioinformatics analyses and explore different research questions related to SCI and baclofen treatment.

Study Limitations

1
The study was conducted on mice, and the results may not directly translate to humans.
2
The RNA-seq data represents a snapshot at 7 days post-injury, limiting insights into longer-term effects.
3
The study focused on a compression SCI model, and the findings may not be generalizable to other types of SCI.

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