Transplantation of Cryopreserved Olfactory Ensheathing Cells Restores Loss of Functions in an Experimental Model

Cell Transplantation, 2023 · DOI: 10.1177/09636897231199319 · Published: August 21, 2023

Spinal Cord Injury Regenerative Medicine & Stem Cells

Simple Explanation

This study investigates whether cryopreserved olfactory ensheathing cells (OECs) can restore neurological function in rats with spinal cord injuries. OECs are cells that promote axon regeneration and remyelination, potentially restoring lost functions. The researchers compared rats that received transplanted cryopreserved OECs to control groups with injuries alone or with collagen gel (but no cells). The results showed that rats with transplanted OECs regained function in a vertical climbing test. This suggests that cryopreserved OECs retain their regenerative properties and could be a viable option for clinical applications, offering a readily available source of cells for transplantation.

Study Duration

6 Weeks

Participants

29 Adult female Sprague–Dawley rats

Evidence Level

Not specified

Key Findings

1
Transplantation of cryopreserved rat olfactory bulb OECs encapsulated in collagen restored the loss of function in a vertical climbing test in a unilateral C6-T1 dorsal root injury model.
2
The loss of function returns in 80% of rats with injuries in about 3 weeks comparable to that observed after transplantation of primary cultured bOECs.
3
Regenerated axons induced by the transplant are identified by neurofilament antibodies and ensheathed by OECs.

Research Summary

This study demonstrates that cryopreserved olfactory ensheathing cells (OECs) can restore lost function in rats with dorsal root injuries, comparable to primary cultured OECs. The transplantation of cryopreserved OECs led to axon regeneration and ensheathment, indicating the cells retained their regenerative properties after cryopreservation. The findings support the potential use of banked cryopreserved allogeneic cells in clinical applications for CNS injuries, offering a readily available cell source.

Practical Implications

Clinical Translation

Cryopreserved allogeneic OECs offer a practical approach for clinical applications by providing an 'off-the-shelf' cell source for transplantation.

Reduced Morbidity

Using banked cells avoids the need for multiple invasive surgeries on patients, reducing associated risks.

Improved Consistency

Allografting banked cells allows for standardized cell quality and quantity, leading to more consistent transplant outcomes.

Study Limitations

1
The study only assessed cryopreservation for one month, limiting conclusions on long-term effects.
2
There was a reduction in the OEC population after cryopreservation.
3
The study did not quantify the diffusion molecular and growth factors.

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