A novel method for obtaining highly enriched Schwann cell populations from mature monkey nerves based on in vitro pre‑degeneration

This study focuses on developing a method to efficiently isolate and purify Schwann cells (SCs) from mature monkey nerves using in vitro pre-degeneration. The process involves culturing nerve pieces in a specialized medium to stimulate SC proliferation and reduce fibroblast migration, followed by enzymatic treatment to dissociate and purify the SCs. The technique uses basic fibroblast growth factor, heregulin, and forskolin to promote SC proliferation during the pre-degeneration phase.

• 1
  In vitro pre-degeneration in a modified SC culture medium (SCCM) increased SC purity and yield from monkey peroneal nerves.
• 2
  Following purification, 96.3±3.9% of cells were identified as SCs, demonstrating high purity using the novel technique.
• 3
  Cell yield was significantly increased following two rounds of purification, reaching 2.1±0.2x106 cells/well.