Biochemical profiling of rat embryonic stem cells grown on electrospun polyester fibers using synchrotron infrared microspectroscopy

This study explores new ways to repair neural tracts in the central nervous system using polymer scaffolds. The scaffolds are made of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) fibers. The behavior of rat neural progenitor cells (NPCs) seeded on these fiber scaffolds was assessed using synchrotron-based infrared microspectroscopy (SIRMS). This technique helped determine the biochemical profiles of NPCs on the polymer fibers. The study found that changes in lipid and amide I–II spectral regions are influenced by the type and coating of the substrate, as well as the culture time. These SIRMS studies can offer valuable insights into how NPCs respond to a biomaterial's morphology and surface chemistry.

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  Changes in lipid and amide I–II spectral regions are modulated by the type and coating of the substrate used and the culture time.
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  The intensities of bands associated with lipid methylene moieties initially increase but then decrease significantly at 48 h.
• 3
  The presence of α-helix structures is favored on ZnS substrates, while β-sheet structures are more likely to be formed on P(HB-co-HHx)/L fibers.