CKB Promotes Mitochondrial ATP Production by Suppressing Permeability Transition Pore

Creatine kinases (CKs) are important for keeping a cell's energy in balance. They move a phosphoryl group from ATP to creatine. This study found that creatine kinases (CKMT1A, CKMT1B, and CKB) are highly expressed in cells that depend on mitochondrial ATP. The study found that silencing CKB leads to a loss of sensitivity to the inhibition of F1F0 ATP synthase in cells, indicating CKB's importance in mitochondrial function. CKB helps mitochondria produce ATP by lowering mitochondrial calcium levels, which prevents the mitochondrial permeability transition pore (mPTP) from activating. This ensures mitochondria can efficiently generate ATP.

• 1
  CKB is essential for maintaining cellular sensitivity to F1F0 ATP synthase inhibition, a key enzyme in mitochondrial ATP production.
• 2
  CKB deficiency impairs mitochondrial ATP production, leading to increased reliance on glycolysis for energy production.
• 3
  CKB suppresses mitochondrial calcium levels and mPTP activity, promoting efficient mitochondrial ATP production.