Comments on “H4K12 Lactylation-­Activated Spp1 in Reprogrammed Microglia Improves Functional Recovery After Spinal Cord Injury”

This letter discusses a study on how a process called H4K12 lactylation can help with recovery after spinal cord injury (SCI) by influencing microglia, which are special cells in the brain and spinal cord. The original study found that a protein called Spp1, when activated by H4K12la, aids in this recovery. The authors suggest confirming the successful overexpression of Spp1 protein by including Western blot results for both the vector group and recombinant Spp1 protein group at multiple time points to verify the expression levels of Spp1, particularly to confirm the duration of stable overexpression. The letter also suggests adding experiments where neurons and microglia are grown together to better understand how Spp1 affects both cell types and contributes to SCI repair, offering a more complete picture of the healing process.

• 1
  The original study demonstrated the significant role of recombinant Spp1 protein (rSPP1) in functional recovery after spinal cord injury.
• 2
  The letter suggests that confirming Spp1 protein expression levels at different time points is essential for understanding its biological effects.
• 3
  The letter proposes that coculture experiments could reveal how Spp1 promotes neuronal recovery by modulating the activation of microglia.