EVAnalyzer: High content imaging for rigorous characterisation of single extracellular vesicles using standard laboratory equipment and a new open-source ImageJ/Fiji plugin

The study introduces EVAnalyzer, an open-source ImageJ/Fiji plugin designed for the automated quantification of single extracellular vesicles (EVs) using fluorescence microscopy images. EVAnalyzer enables researchers to perform quantitative single vesicle analysis using standard laboratory equipment and open-access software, making this technology more accessible. The plugin facilitates the characterization of EV subpopulations, validation of EV labeling reagents, determination of antibody specificity, and optimization of genetic EV engineering.

• 1
  EVAnalyzer enables the automated and quantitative analysis of single vesicles from imaging data, using a robust protocol for capture, labeling, and fluorescent imaging of EVs.
• 2
  The plugin can be used to quantify EV subpopulations, validate EV labeling reagents, determine antibody specificity, and optimize genetic EV engineering.
• 3
  EVAnalyzer is applicable to synthetic nanoparticles, allowing the determination of siRNA encapsulation efficiencies of lipid-based nanoparticles and protein loading of SiO2 nanoparticles.