Immunohistochemical profile of ING3 protein in normal and cancerous tissues

ONCOLOGY LETTERS, 2017 · DOI: 10.3892/ol.2017.5632 · Published: January 1, 2017

Simple Explanation

This study examines the expression of ING3 protein in various normal and cancerous tissues in mice and humans. ING3 is a protein that can block the cell cycle and induce apoptosis (cell death). The researchers used immunohistochemistry to identify where ING3 protein is located within different tissues. They found that ING3 is present in both the cytoplasm and nucleus of various cells, with stronger presence in stomach, skin, and cervical tissues. In cancerous tissues, ING3 expression was most frequent in gynecological cancers such as ovarian, endometrial, breast, and cervical cancers. This suggests a potential role of ING3 in the development and progression of these cancers.

Study Duration

Not specified

Participants

Mouse and human normal tissue, human hepatocellular (n=62), renal clear cell (n=62), pancreatic (n=62), esophageal squamous cell (n=45), cervical squamous cell (n=31), breast (n=144), gastric (n=196), colorectal (n=96), ovarian (n=208), endometrial (n=96) and lung carcinoma (n=192)

Evidence Level

Not specified

Key Findings

1
ING3 protein is widely distributed in normal mouse tissues, particularly in the cytoplasm of cardiomyocytes, kidney, and skeletal muscle cells.
2
In normal human tissues, ING3 protein is primarily located in the cytoplasm but also found in the nucleus of cells in the tongue, esophagus, stomach, and other tissues.
3
ING3 expression is most frequently observed in gynecological cancers, suggesting a potential link between ING3 and the development of these cancers.

Research Summary

The study investigates the expression profile of ING3 protein in both normal and cancerous tissues of mice and humans, using immunohistochemistry to determine its location within cells. Results indicate that ING3 is present in various normal tissues, with a strong presence in stomach, skin, and cervical cells. In cancer tissues, ING3 is more frequently expressed in gynecological cancers. The findings suggest that ING3 may play a role in tissue repair and regeneration, as well as in gynecological carcinogenesis, warranting further investigation into its specific functions in these processes.

Practical Implications

Diagnostic Marker

ING3 expression levels could potentially be used as a diagnostic marker, particularly for gynecological cancers.

Therapeutic Target

Targeting ING3 expression or function may offer a novel approach to treating cancers, especially those where ING3 is downregulated.

Further Research

Further research is needed to clarify the specific mechanisms by which ING3 influences cancer development and progression.

Study Limitations

1
The study relies on immunohistochemistry, which provides a snapshot of ING3 expression at a single time point.
2
The study does not investigate the functional consequences of altered ING3 expression in cancer cells.
3
The mechanisms that regulate ING3 localization and expression remain unclear.

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