In vivo Electroporation of Morpholinos into the Regenerating Adult Zebrafish Tail Fin

This paper describes a method to efficiently introduce fluorescein-tagged antisense morpholinos into regenerating zebrafish fins to knockdown expression of the target protein. The morpholino is micro-injected into each blastema of the regenerating zebrafish tail fin and electroporated into the surrounding cells. Fluorescein provides the charge to electroporate the morpholino and to visualize the morpholino in the fin tissue.

• 1
  The described protocol permits conditional protein knockdown to examine the role of specific proteins during regenerative fin outgrowth.
• 2
  The technique can be adapted to study the role of specific proteins during wound healing or blastema formation.
• 3
  The technique can be used as a potential marker of cell migration during blastema formation.