Plasmid Releasing Multiple Channel Bridges for Transgene Expression After Spinal Cord Injury

This study explores a novel approach to spinal cord regeneration using plasmid-loaded multiple channel bridges. These bridges are designed to support cell growth and deliver genes directly to the injury site. The goal is to create an environment that encourages nerve regeneration by combining physical support with gene therapy. The bridges are made with controlled pore sizes to optimize gene expression. Experiments showed that larger pore sizes led to more consistent and sustained gene expression. In a rat spinal cord injury model, the bridges supported cell infiltration and axon growth. The transfected cells at the implant site were identified as Schwann cells, fibroblasts, and macrophages. The study suggests that combining gene delivery with a supportive scaffold structure could be a promising strategy for regenerating complex tissues like the spinal cord.

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  Plasmid-loaded bridges support substantial cell infiltration and aligned cells within the channels.
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  Axon growth across the channels was observed, indicating the bridge's ability to guide nerve regeneration.
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  Transgene expression was high at the implant site, with decreasing levels away from it, suggesting localized gene delivery.