Proseek single-plex protein assay kit system to detect sAxl and Gas6 in serological material of brain tumor patients

Biotechnology Reports, 2018 · DOI: 10.1016/j.btre.2018.e00252 · Published: January 1, 2018

Simple Explanation

The study investigates the use of the Proseek Single-Plex Protein Assay Kit System to detect sAxl and Gas6 in blood samples from patients with high-grade glioma (HGG). Axl and Gas6 are proteins involved in HGG pathogenesis. The Proseek method is compared to the standard ELISA method for measuring protein levels. The Proseek assay provides results within 24 h without washing steps, and only 1 ml of serum is needed. The Proseek method combines oligonucleotide-labeled antibodies with PCR amplification to quantify proteins with high sensitivity and specificity, potentially visualizing low protein levels even before tumors are detected by imaging.

Study Duration

Not specified

Participants

3 patients with high-grade glioma and 1 healthy control

Evidence Level

Not specified

Key Findings

1
The Proseek method demonstrates higher sensitivity in detecting lower concentrations of Gas6 compared to the DuoSet sandwich ELISA method.
2
The Proseek method is more sensitive in detecting low concentrations of sAxl compared to ELISA detection.
3
Elevated levels of sAxl concentration in serum were observed in some patients with HGG compared to a healthy person, with variations observed during anti-angiogenic therapy.

Research Summary

This study explores the use of the Proseek Single-Plex Protein Assay Kit System for detecting sAxl and Gas6 in HGG patients' blood, aiming for more sensitive detection than standard ELISA methods. The Proseek method showed greater sensitivity in detecting low concentrations of both Gas6 and sAxl compared to ELISA, suggesting its potential for improved monitoring of these proteins in HGG. The study suggests that the Proseek Multi-Plex Protein Assay Kit system could be a valuable tool for further analysis of sAxl and Gas6 in relation to HGG progression.

Practical Implications

Improved HGG Diagnosis

The Proseek assay's high sensitivity could lead to earlier detection of HGG recurrence or progression compared to traditional methods.

Personalized Therapy Monitoring

Monitoring sAxl and Gas6 levels with Proseek during Bevacizumab treatment could help tailor therapy based on individual patient responses.

Enhanced Research Capabilities

The Proseek method's ability to work with small sample volumes makes it useful for analyzing precious biobanked material or paraffin-embedded tissues.

Study Limitations

1
The primary antibody has to be compatible and well selected which suits to the assay
2
the method needs some time for establishment to ensure precise detections (e.g. DNA ligation, real time-PCR)
3
method needs some time for establishment to ensure precise detections

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