The effects of local delivery of laurus nobilis extract and adipose derived stem cells via electrospun gelatin scaffold on spinal cord injury inﬂammatoradscy response and its regeneration

Regenerative Therapy, 2024 · DOI: https://doi.org/10.1016/j.reth.2024.09.008 · Published: September 23, 2024

Spinal Cord Injury Pharmacology Regenerative Medicine & Stem Cells

Simple Explanation

This study explores using gelatin nanofibers loaded with Laurus nobilis extract to deliver adipose-derived stem cells (ADSCs) to treat spinal cord injury in rats. The delivery system was tested in vitro for biocompatibility and anti-inflammatory properties. In vivo, rats treated with the system showed improved functional recovery and reduced inflammation. The findings suggest this delivery system could improve clinical outcomes for spinal cord injury by enhancing recovery and reducing inflammation.

Study Duration

8 weeks

Participants

15 male Wistar rats

Evidence Level

Not specified

Key Findings

1
Laurus nobilis extract-loaded scaffolds reduced tissue expression levels of pro-inflammatory cytokines like TNF-a, IL-1b, and IL-6.
2
Rats treated with Laurus nobilis extract and ADSCs loaded scaffolds exhibited heightened functional recovery (BBB score of 14.66 ± 1.52 % and hot plate latency time of 8.33 0.26 s).
3
Histological restoration was observed at the 8-week mark post-injury in rats treated with Laurus nobilis extract and ADSCs loaded scaffolds.

Research Summary

This study investigates a novel approach to spinal cord injury treatment by combining the anti-inflammatory properties of Laurus nobilis extract (LNE) with the regenerative capabilities of adipose-derived stem cells (ADSCs). The LNE and ADSCs were delivered via electrospun gelatin scaffolds, and the results showed improved functional recovery and histological restoration in a rat model of spinal cord injury. The study highlights the potential of this delivery system to reduce inflammation and enhance tissue repair, offering a promising therapeutic strategy for spinal cord injury.

Practical Implications

Novel Therapeutic Strategy

The combination of LNE and ADSCs delivered via electrospun gelatin scaffolds represents a novel therapeutic strategy for SCI.

Clinical Translation Potential

The findings suggest a potential pathway for developing new, natural-based therapies for SCI, with possible applications to other inflammatory or degenerative conditions.

Enhanced Functional Recovery

The improved functional recovery and reduced inflammation observed in the study indicate a promising approach to enhance clinical outcomes in SCI.

Study Limitations

1
The study was conducted on a rat model, and the results may not be directly translatable to humans.
2
Further research is needed to optimize the strategy in larger animal models before human clinical trials.
3
Long-term studies are needed to evaluate the durability and sustained effectiveness of the treatment.

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