Genetics Research

KEY FINDING: This protocol provides a method for preparing mixed glial cells from adult mouse spinal cord, suitable for in vitro evaluations or further preparation of microglia-enriched and microglia-depleted cells.

KEY FINDING: Spinal cord ischemia/reperfusion injury increased EphA4 expression in the neurons of anterior horn and markedly worsened ferroptosis-related indicators.

KEY FINDING: Zinc treatment significantly increased LC3B, PINK1, and Mito-­Parkin levels after spinal cord injury, suggesting an upregulation of mitophagy.

KEY FINDING: BoNTs inhibit the release of neurotransmitters involved in the pain pathway, including CGRP, glutamate, substance P, and ATP, by suppressing SNARE proteins.

KEY FINDING: EPT treatment led to a significant increase in NeuN-positive cells 28 days after SCI in EPT-treated animals, indicating reduced neuronal loss.

KEY FINDING: DTMP reversed expression levels of 83% of proteins affected by the pain model back to levels seen in uninjured animals, whereas a low-rate (LR-SCS) approach reversed 67%.

KEY FINDING: Edema formation is one of the first events of the acute phase of spinal cord injury (SCI) and its mechanisms are similar in the brain and spinal cord, but structural and tissue-specific differences can affect edema formation in these two regions.

KEY FINDING: PTEN-KO using AAVrg’s improves locomotor outcomes in both acute and chronic SCI conditions, however, the effects are severity dependent.

KEY FINDING: Peripheral nerve injury downregulates ciRNA-Kat6b in the dorsal spinal horn of male mice, and rescuing this downregulation alleviates CCI-induced pain hypersensitivities.

KEY FINDING: Knockdown of spastin inhibits HIV-1 Gag protein production and new virion formation by enhancing Gag lysosomal degradation.